LOCUS pHIS8_4_Lucifera 6996 bp ds-DNA circular 19-FEB-2015 DEFINITION Complementary copy of pET-28a(+). SOURCE ORGANISM COMMENT http://www.informaxinc.com COMMENT ORIGDB|GenBank COMMENT VNTDATE|532134159| COMMENT VNTDBDATE|532134296| COMMENT VNTNAME|pHIS8-3| COMMENT VNTAUTHORNAME|Demo User| COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 2920..2944 /dnas_title="lac operator" /vntifkey="4" /label=lac operator /ApEinfo_fwdcolor=#ffa249 /ApEinfo_revcolor=#4f86ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 4925..5380 /dnas_title="f1 origin" /vntifkey="33" /label=f1 origin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 source 3051..4703 /organism="Photinus pyralis" /mol_type="mRNA" /db_xref="taxon:7054" /country="USA: Illinoi, Palos Forest" /collection_date="27-May-2008" /collected_by="Yuichi Oba" /identified_by="Marc A. Branham" /label=source:Photinus pyralis /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(5476..6288) /dnas_title="kan sequence" /vntifkey="4" /label=kan sequence /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 1..1 /dnas_title="ColE1 pBR322 origin" /vntifkey="33" /label=ColE1 pBR322 origin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(1435..2514) /dnas_title="lac I" /vntifkey="4" /label=lac I /ApEinfo_fwdcolor=#ff8d89 /ApEinfo_revcolor=#ff728a /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3051..4703 /gene="PpyLuc1" /codon_start=1 /product="firefly luciferase" /protein_id="BAL46512.1" /db_xref="GI:374081834" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" /label=firefly luciferase /ApEinfo_fwdcolor=#ccff00 /ApEinfo_revcolor=#b7ff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 terminator 4842..4888 /dnas_title="T7 terminator" /vntifkey="43" /label=T7 terminator /ApEinfo_fwdcolor=#ff1ece /ApEinfo_revcolor=#34ff2b /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 protein_bind 4757..4774 /dnas_title="His tag" /vntifkey="31" /label=His tag /ApEinfo_fwdcolor=#1db0ff /ApEinfo_revcolor=#ff2d37 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 2901..2917 /dnas_title="T7 promoter" /vntifkey="30" /label=T7 promoter /ApEinfo_fwdcolor=#f9ff4a /ApEinfo_revcolor=#5769ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 RBS 2975..2980 /dnas_title="rbs" /vntifkey="32" /label=ribosomal binding site (rbs) /ApEinfo_fwdcolor=#5dff56 /ApEinfo_revcolor=#ff5ff3 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2994..3017 /dnas_title="N-His tag" /vntifkey="21" /label=N-His tag /ApEinfo_fwdcolor=#43c1ff /ApEinfo_revcolor=#ff6561 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2988..2990 /note="Start Codon" /label=Start Codon /ApEinfo_fwdcolor=#39ff33 /ApEinfo_revcolor=#ff48ea /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3024..3044 /note="TE Protease Site" /label=TE Protease Site /ApEinfo_fwdcolor=#7fffb5 /ApEinfo_revcolor=#ff65f5 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3044..3049 /note="NcoI Diagnostic Digest Site" /label=NcoI Diagnostic Digest Site /ApEinfo_fwdcolor=#ff000a /ApEinfo_revcolor=#00ffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 61 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 181 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat 241 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg 301 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg 361 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg 421 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa 481 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc 541 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg 601 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg 661 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat 721 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag 781 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag 841 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc 901 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc 961 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc 1021 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg 1081 cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg 1141 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac 1201 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct 1261 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata 1321 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt 1381 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc 1441 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 1501 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa 1561 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt 1621 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct 1681 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc 1741 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg 1801 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc 1861 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc 1921 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg 1981 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat 2041 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc 2101 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac 2161 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac 2221 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat 2281 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt 2341 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc 2401 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt 2461 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac 2521 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca 2581 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc 2641 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga 2701 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc 2761 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc 2821 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg 2881 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa 2941 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg aaacaccacc 3001 accaccacca ccaccacggt ggtGAAAACT TGTACTTCCA GGCCcatggc atggaagacg 3061 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag 3121 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag 3181 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg 3241 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa 3301 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc 3361 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta 3421 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac 3481 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga 3541 tgtacacgtt cgtcacgtct catctacctc ccggttttaa tgaatacgat tttgtaccag 3601 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctggg tctactgggt 3661 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag 3721 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc 3781 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct 3841 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa 3901 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat 3961 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg 4021 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg 4081 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta 4141 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg 4201 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca 4261 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag 4321 cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat 4381 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca 4441 tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg 4501 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca 4561 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga 4621 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga 4681 agggcggaaa gtccaaattg taaccatggc ggatccgaat tcgagctccg tcgacaagct 4741 tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg 4801 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc 4861 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa 4921 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 4981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 5041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 5101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 5161 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 5221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 5281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 5341 tttaacgcga attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga 5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 5461 atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc 5521 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc 5581 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac 5641 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc 5701 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg 5761 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt 5821 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca 5881 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc 5941 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag 6001 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc 6061 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc 6121 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc 6181 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga 6241 atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt 6301 attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct taacgtgagt 6361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 6421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 6481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 6541 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 6601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 6661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 6721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 6781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 6841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 6901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 6961 ttttgtgatg ctcgtcaggg gggcggagcc tatgga //